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Image Search Results
Journal: Biochimica et Biophysica Acta. Bioenergetics
Article Title: The ASCT/SCS cycle fuels mitochondrial ATP and acetate production in Trypanosoma brucei
doi: 10.1016/j.bbabio.2020.148283
Figure Lengend Snippet: Reaction mechanism of TbASCT. The double reciprocal plot of varying the concentration of (A) acetyl-CoA under a set of fixed succinate concentrations of 5, 10, and 20 mM and varying the concentration of (B) succinate under a set of fixed acetyl-CoA concentrations of 0.02, 0.05, and 0.1 mM. (C) Schematic representation of ping-pong bi-bi mechanism catalyzed by TbASCT. (D) BN-PAGE and In-gel ASCT activity staining of TbASCT, M: Protein marker. Major bands identified from both staining are shown in red arrows with calculated molecular weight of 225, 414 and 564 kDa, which corresponds to one, two or three tetramers of TbASCT, respectively.
Article Snippet: The
Techniques: Concentration Assay, Activity Assay, Staining, Marker, Molecular Weight